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CHAPTER 21 - TISSUE LOOK ALIKES
Histology Guide
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MICROSCOPE SLIDE

SLIDE NAME
Look Alike #1
TISSUE
MH 109 Esophagus
MH 109 Esophagus
MH 173 Vagina
STAIN
Hematoxylin & Eosin
IMAGE SIZE
55,961 x 22,904 pixels
4.4 GB
FILE SIZE
153 MB
OBJECTIVE
40x
PIXEL SIZE
0.3171 µm
SOURCE
Department of Genetics, Cell Biology, and Development
School of Medicine
University of Minnesota
Minneapolis, MN

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Robert L. Sorenson, Ph.D.

Professor Emeritus
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

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Tissue Look Alikes #1

Left Tissue Section

These tissues are often confused for each other - can you identify them?

This slide is an example of the type of questions one might ask when trying to identify these tissues.

Examine the uppermost surface of the tissue. What type of epithelium is seen? [ + ]

Stratified squamous non-keratinized epithelium - must be a moist surface

What layers are visible in this tissue? [ + ]

  • Stratified squamous non-keratinized epithelium
  • Loose connective tissue
  • Band of smooth muscle
  • Dense irregular connective tissue
  • Thick layer of muscle (inner - circular, outer - longitudinal)

The wall of what tissues has these features? [ + ]

GI tract – the layers are:
  • Mucosa (epithelium, lamina propria, and muscularis mucosae)
  • Submucosa
  • Muscularis externa

Where does a stratified squamous non-keratinized epithelium occur in the GI tract? [ + ]

Esophagus
(MH 109 Esophagus)

Middle Tissue Section

Does it look like the left section? [ + ]

Yes, except for the presence of mucous glands (muscularis externa is also missing)

Where in the GI tract do glands occur in the submucosa? [ + ]

Esophagus and duodenum

Which has a stratified squamous non-keratinized epithelium? [ + ]

Esophagus
(MH 109 Esophagus)
[duodenum has villi with a simple columnar epithelium]

Right Tissue Section

Examine the surface of the section. What type of epithelium is it? [ + ]

Stratified squamous non-keratinized epithelium - must be a moist surface

What type of tissue underlays the epithelium? [ + ]

  • Lamina propria
  • Two thick, indistinct layers of smooth muscle (inner - circular, outer - longitudinal)

Absence of muscularis mucosae - not part of the GI tract (in particular, the esophagus)

Where does this occur? [ + ]

Vagina
(MH 173 Vagina)
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