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CHAPTER 4 - MUSCLE TISSUE
Histology Guide
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MICROSCOPE SLIDE

SLIDE NAME
MH 055ahr Skeletal Muscle
TISSUE
Skeletal Muscle
(human)
STAIN
Hematoxylin & Eosin
FIXATIVE
Zenker's Formaldehyde
IMAGE SIZE
8,598 x 6,708 pixels
215 MB
FILE SIZE
14 MB
OBJECTIVE
60x
(oil immersion objective)
PIXEL SIZE
0.0976 µm
SOURCE
Department of Genetics, Cell Biology, and Development
School of Medicine
University of Minnesota
Minneapolis, MN

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University of Minnesota
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Robert L. Sorenson, Ph.D.

Professor Emeritus
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

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MH 055ahr Skeletal Muscle

Skeletal Muscle

This image was obtained from a longitudinal section of skeletal muscle using a 60x oil immersion objective. The increased resolution is helpful in viewing sarcomeres.

Skeletal muscle cells (or fibers) are filled with tightly packed myofibrils. Myofibrils show an alternating series of striations due to the repeating sarcomeres. Each sarcomere is 2 to 3 µm long and extends from one Z line to the next.

Look for areas (,,) where striations can be identified. Identify the bands in this higher magnification image of several sarcomeres.

  • A band - the main dark band
  • I band - the main light band
  • Z line - thin dark line in the middle of the I band
  • H band - thin light band in the middle of the A band

These striations are seen with higher resolution in EM 222 Skeletal Muscle and EM 123 Skeletal Muscle by transmission electron microscopy.

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