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CHAPTER 4 - MUSCLE TISSUE
Histology Guide
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MICROSCOPE SLIDE

SLIDE NAME
MH 055a Skeletal Muscle
TISSUE
Skeletal Muscle
(human)
STAIN
Hematoxylin & Eosin
FIXATIVE
Zenker's Formaldehyde
IMAGE SIZE
88,270 x 76,994 pixels
25 GB
FILE SIZE
748 MB
OBJECTIVE
40x
PIXEL SIZE
0.3171 µm
SOURCE
Department of Genetics, Cell Biology, and Development
School of Medicine
University of Minnesota
Minneapolis, MN

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Robert L. Sorenson, Ph.D.

Professor Emeritus
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

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MH 055a Skeletal Muscle

Skeletal Muscle

Skeletal muscle fibers are elongated cylindrical-shaped cells with multiple, peripherally located nuclei and a cytoplasm filled with contractile filaments.

    • - have polygonal cross-sections (50 to 100 µm in diameter) with nuclei at the periphery
    • Skeletal Muscle Cells - can vary in length from a few millimeters to almost a meter
      • - the cytoplasm is filled with myofibrils that extend the entire length of the cell. Individual myofibrils are only seen where they are slightly separated.
      • - myofibrils show an alternating series of striations due to the repeating sarcomeres. (Shown better in the next two slides.)
        • A band - the main dark band
        • I band - the main light band
        • H band - thin light band in the middle of the A band
        • Z band - thin dark line in the middle of the I band
  • - occur on the surface of muscle cells. Only their small nuclei of mostly heterochromatin can be identified
  • - a collapsed capillary containing flattened red blood cells is present on the left edge of the center muscle cell
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